2008•Unpublished venueRequires access
The influence of transfusion of donor apoptotic cells on the function of islet grafts in rat islet transplantation
Hua Zhang
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Abstract
Objective To observe the influence of transfusion of donor apoptotic cells on the function of islet grafts in rat islet transplantation.Methods Recipient SD rats were rendered diabetic by a single intraperitoneal injection of streptozotocin(STZ).Donor Wistar rat apoptotic splenocytes were induced by X-irradiation with electron linear accelerator.The donor apoptotic splenocytes were injected into the recipient SD rats via dorsal veins of penis.Diabetic SD rats received islets transplantation under their renal capsule from the same donor rat 7 days later.The survival time and insulin level were examined to reflect the function of islet grafts.Results Pre-infusion of donor apoptotic splenocytes significantly prolonged allograft survival time(MST 31 d).Serum insulin level in diabetic SD rats had a significant increase at 7d [(19.26±4.50)mIU/ml] and 14 d[(20.70±6.33)mIU/ml] after transplantation in apoptotic group and decreased after rejection.Conclusion Transfusion of donor apoptotic splenocytes prolongs the survival and normal function of islet graft.