Mutagenic Effect of Daily Articles on Root Tip Cells of Vicia faba
Shao Ai-hua
Abstract
Shao Ai-hua
Abstract
[Objective] The aim was to research the mutagenic effect of common daily articles on market on root tip cells of Vicia faba L.[Method] Three kinds of daily articles(Floral water,detergent and toothpaste) with different concn.were selected as mutagen to treat the root tip cells of Songzi black V.faba resp.and the micronucleus rate of V.faba root tip cells was determined by micronucleus test.[Result] After V.faba root tip cells were treated by treatment liquor of Floral water,detergent and toothpaste with different concn.,the shape of V.faba root tip cells was damaged to different extent,the root tip length was shortened obviously,and changed to yellow and black.The micronucleus mutation test showed that the V.faba root tip cells treated by daily articles with different concn.could induce micronucleus rate with different frequency,which presented increasing tendency with the raise of treatment concn.in a certain range.When the concn.of detergent treatment group reached to the stock solution one,the micronucleus rate showed decline trend,but was still higher than that obtained from the negative control group with distilled water.[Conclusion] The daily articles had some certain inducing effect on V.faba root tip cells.
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[Objective] The aim was to research the mutagenic effect of common daily articles on market on root tip cells of Vicia faba L.[Method] Three kinds of daily articles(Floral water,detergent and toothpaste) with different concn.were selected as mutagen to treat the root tip cells of Songzi black V.faba resp.and the micronucleus rate of V.faba root tip cells was determined by micronucleus test.[Result] After V.faba root tip cells were treated by treatment liquor of Floral water,detergent and toothpaste with different concn.,the shape of V.faba root tip cells was damaged to different extent,the root tip length was shortened obviously,and changed to yellow and black.The micronucleus mutation test showed that the V.faba root tip cells treated by daily articles with different concn.could induce micronucleus rate with different frequency,which presented increasing tendency with the raise of treatment concn.in a certain range.When the concn.of detergent treatment group reached to the stock solution one,the micronucleus rate showed decline trend,but was still higher than that obtained from the negative control group with distilled water.[Conclusion] The daily articles had some certain inducing effect on V.faba root tip cells.
Key concepts: Vicia faba, Root tip, Micronucleus test, Micronucleus, Distilled water, Horticulture, Mutagen, Biology