Identification of a Sex-associated RAPD Marker in Cycas tanqingii D. Y. Wang

The total genomic DNA was extracted from half-a-year-old leaflets of the male and female cycads (Cycas tanqingii D. Y. Wang) using an optimized CTAB protocol. The RAPD-PCR amplification conditions were optimized by monofactor and multifactor orthogonal experiments. A total of 160 primers were used for RAPD-PCR amplification and more than 1 450 fragments were obtained from both male and female plants. A fragment of about 500 bp only linked to the female was generated by the S0465 primer, which can be used in sex determination of C. tanqingii.