The effect of suppressive oligodeoxynucleotide on pSTAT4,pSTAT6 and Tbet expression of concanavalin A-induced liver injury in micee

Objective To investigate the effect of suppressive oligodeoxynucleotide(Sup ODN)on pSTAT4, pSTAT6 and T-bet expression of concanavalin A induced liver injury in mice.Methods Forty Balb/C mice were randomly divided into 4 groups,normal saline group(NS,blank control group),cyclosporin A group(CsA group, positive control group),control oligodeoxynucleotide group(control ODN group),and Sup ODN group(trial group).Mice in each group were injected with ConA(20mg/kg)via the tail vein to make liver injury model.Sup ODN,Con ODN,CsA or NS was injected intraperitoneally at the same time of ConA injection.The expression of pSTAT4,pSTAT6 and T-bet in spleen at the 8th or 24th hour after intraperitoneal injection was examined by Western-blotting. Results pSTAT4 and T-bet in Sup ODN group were significantly lower compared with NS,Con ODN or CsA group respectively(all P0.01);pSTAT6 had no difference between Sup ODN group and other groups( all P0.01).Conclusion Sup ODN attained protective effect on ConA induced mice liver injury by supressing expression of pSTAT4 and T-bet.