Studies on mechanism of proacrosin activation of capacitation and frozen-thawed boar spermatozoa

With SDS-PAGE and Western Blotting methods to analysis proacrosin conversion process of frozen-thawed boar sperm.The results showed that fluorescence region the capacitation group sperm is greater than the frozen-thawed group sperm,while less than fresh group sperm.Fresh group sperm have 45 kDa and 35 kDa two bands,and frozen-thawed and capacitation sperm have 45 kDa,35 kDa and 28 kDa three bands.The results indicated part of the proacrosin are activated into α-acrosin,β-acrosin and 28 kDa protein expression during capacitation and freezing-thawing of boar spermatozoa.In conclusion,acrosomal integrity of boar sperm can be normal fertilization except for capacitation-like sperm after freezing and thawing.