Effect of Combination Treatment with Growth Horrmone and Glutamine Enriched Total Parenteral Nutrition on Intestinal Mucosal Epithelial Cell Proliferation in Rats with Small Bowel Syndrome

Purpose To explore the effect of combination treatment with growth hormone(GH) and glutamine(Gln) enriched total parenteral nutrition(PN) on intestinal mucosal epithelial cell proliferation. Methods Forty Spragur Dawley rats with small bowel syndrome were randomly divided into four groups according to 2×2 factorial experiment and were treated with standard PN(S?PN group, n =10),PN+Gln(Gln group, n =10),PN+GH(GH group, n =10) and PN+Gln+GH(GG group, n =10) respectively for six days,Eight normal sham operation rats served as baseline controls(control group, n =8).DNA content and expression of PCNA of intestinal mucosal epithelial cells were contrastly studied among those groups by using image analysis and immunohistochemical technique. Results Gln or GH administration significantly increased intestinal mucosal epithelial cells DNA content,percentage of S phase and expression of PCNA,But combined Gln and GH had better effect than Gln or GH administration individually( P 0.01). Conclusions Combination treatment with growth hormone and glutamine enriched total parenteral nutrition can promote intestinal mucosal epithelial cell proliferation to prevent intestinal mucosal atrophy.