The early protective effects and mechanism of prostaglandin E1 pretreatment on myocardial injury induced by ischemia reperfusion in rabbits hearts.

Objective: To observe the early protective effect of prostaglandin E1(PGE1) on myocardial ischemia reperfusion through the establishment of rabbit model and investigate the preliminary mechanism.Methods: Forty Japanese white rabbits were divided into four groups: ischemia-reperfusion group(IR),ischemic preconditioning group(IP),alprostadil preconditioning group(PGE1) and alprostadil combined with Glibenclamide(GLI) group.The creatine kinase isoenzyme(CK-MB),troponin I(cTnI),superoxide dismutase(SOD) and malondialdehyde(MDA) in rabbit serum of each group were measured.The size of myocardial infarction was measured by pathological examination.Myocardial histological morphology was observed by microscope.Results: The CK-MB,cTnI and MDA were significantly decreased and the SOD was significantly increased in IP group and PGE1 group compared with those in IR group(P0.05).But there was no significant difference between GLI group and IR group(P0.05).The size of myocardial infarction was significantly large in IP group and PGE1 group compared with that in IR group(P0.05).No significant difference was observed between GLI group and IR group(P0.05).Conclusion: The range of myocardial infarction and serum CK-MB,cTnI leakage reduced after alprostadil preconditioning,which improved cardiac antioxidant capacity,simulated the ischemic preconditioning,and effectively protected the acute myocardial ischemia reperfusion injury,meanwhile,preconditioning protection role should be actived by KATPchannel openning up.Therefore,alprostadil preconditioning combinated with coronary artery bypass,cardiopulmonary bypass,cardiac valve replacement and percutaneous transluminal coronary angioplasty have an optimistic outlook in the future and widely used in clinical practice.