Expressions of KISS-1 and MMP-9 in esophageal squamous cell carcinoma tissue

Aim:To explore the relationship between metastasis suppressor gene KISS-1, matrix metalloproteinase-9 (MMP-9) and the invasion, metastasis of esophageal squamous cell carcinoma.Methods:The expressions of KISS-1 and MMP-9 protein and mRNA in specimens taken from 50 cases of esophageal carcinoma and 50 cases of normal esophageal mucosa were detected by immunohistochemical assay (SP) and reverse transcription-polymerase chain reaction (RT-PCR). To analyze the relationship between them and clinical data.Results: The positive expression rate of KISS-1 protein in esophageal squamous cell carcinoma (61.0%) was significantly lower than that in normal esophageal mucosa (82.0%) (P0.05). The low expression of KISS-1 protein in esophageal squamous cell carcinoma was related with lymph node metastasis (P0.05).The positive expression rate of MMP-9 protein in esophageal squamous cell carcinoma (72.0%) was significantly higher than that in normal esophageal mucosa (52.0%) (P0.05). The high expression of MMP-9 protein in esophageal squamous cell carcinoma was related with differentiation and the depth of invasion and the metastasis of lymph node (P0.05).The positive expression rate and relative contents of KISS-1 mRNA in esophageal squamous cell carcinoma (56.0%, 0.992±0.090) were significantly lower than those in normal esophageal mucosa (88.0%, 1.075±0.181) (P0.05). The low expression of KISS-1 mRNA in esophageal squamous cell carcinoma was related with lymph node metastasis (P0.05).The positive expression rate and relative contents of MMP-9 mRNA in esophageal squamous cell carcinoma (72.0%, 1.030±0.153) were significantly higher than those in normal esophageal mucosa (44.0%, 0.943±0.085) (P0.05). The high expression of MMP-9 mRNA in esophageal squamous cell carcinoma was related with the depth of invasion and the metastasis of lymph node(P0.05). The expression of KISS-1 had negative correlation with the expression of MMP-9(P0.05).Conclusion: Low expression of KISS-1 and high expression of MMP-9 may be related with invasion and metastasis of esophageal carcinoma. KISS-1 and MMP-9 could be a marker to evaluate invasion and metastasis in esophageal squamous cell carcinoma.