The effect of ScFv against CTGF on bleomycin-induced pulmonary fibrosis of mice and hydroxyproline content

Objective: To investigate therapeutic effect of single-chain variable fragment(ScFv) against connective tissue grouth factor(CTGF) on bleomycin-induced pulmonary fibrosis of Kunming mice.Methods: 36 Kunming mice were randomly divided into three groups: the control group,the model group and the treatment group,12 rats in each group.Mice in the control group were injected with normal saline via trachea,mice in the model and treatment group were injected with bleomycin(5 mg·kg-1) via trachea for preparing models.Then,mice the treatment group were injected with ScFv(4 mg·kg-1) via intravenous,and the mice in the other groups were injected with normal saline(about 100 μl·time-1)via intravenous,3 times·week-1.After be modeled 14 and 28 days,dealing with animals by hematoxylin-eosin staining for histopathological examination and fibrosis grade,by acid hydrolysis for assaying lung hydroxyproline content.Results: There was no pulmonary fibrosis in the control group;in the model group,a large number of alveolus damaged,alveolar septum fibrosis,hydroxyproline content and fibrosis were significantly higher than that in the control group(P0.01);in the treatment group,lung fibrosis was significantly alleviated and the content of hydroxyproline was significantly lower than that in the model group(P0.01).Conclusion: ScFv against CTGF can delay the fibrosis,which may become a new cure for pulmonary fibrosis.