Preparation and Pharmacodynamics Study of a Novel Rapid-Acting Analog of Human Insulin

A novel rapid-acting analog of human insulin named PInsulin(PIns) was prepared,and its molecular weight was characterized by Autoflex ToF/ToF(Bruker Daitonics) mass spectrum,and the concentration of PIns was determined by Folin-phenol reagent method.Its pharmacodynamics was compared with human insulin and insulin Lispro by experiment carried out on male Sprague-Dawley rats.The self-association of PIns was also determined.The results showed that the Mr of PIns was 6286.50,and the concentration of PIns was 0.227mg/ml.The experiment carried out on rats after intramuscular administration of PIns showed that time needed for the lowest percentage of blood glucose concentration(BGC) to emerge was significantly less than that with human insulin(P0.01).Same observation was made for the time for BGC to reach again its level as before injection(P0.01);and theminor percentage of BGC was almost same as human insulin.The self-association of PIns was further studied by size-exclusion chromatography with a longer retention time than that of human insulin,and so the monomeric property of PIns is stronger than that of human insulin.PIns is a promising novel rapid-acting analog of human insulin.