2015•Jilin Nongye Daxue xuebaoRequires access
Expression and Function of Cryptosporidium parvum TSP3 Protein
Sun Tin
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Abstract
By cloning Cryptosporidium parvum TSP3,prokaryotic expression vector was constructed,and to immunize New Zealand rabbits,TSP3-His tag of recombinant protein was purified. By indirect ELISA method,specific serum titers were detected and polyclonal antibodies were purified. The purified TSP3-GST tag of recombinant protein was incubated with HCT-8 cells by Western blot and indirect ELISA cell adhesion properties. This study successfully constructed Cryptosporidium parvum p ET-TSP3 and p GEX-TSP3 recombinant plasmid,and by affinity chromatography successfully purified recombinant proteins of high quality. Cp TSP3 recombinant proteins are of good immunogenicity and can bind HCT-8.