2010•Zhonghua zhongyiyao xuekanRequires access
Effects of Curcumin on the Proliferation and Apoptosis in A549 Lung Cancer Cells
Kechun Liu
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Abstract
Objective: To explore the effects of curcumin on the proliferation and apoptosis in A549 lung cancer cells.Methods: The cell viability was determined by MTT assay.Cell morphology and nuclear fragmentation were analyzed by acridine orange(AO) staining under fluorescent microscope.Cell apoptosis was detected by TUNEL assay.The content of P53 protein was analyzed by western blot assay.Results: Curcumin significantly inhibited A549 cell viability in a dose and time-dependent manner.The changes of morphology associated with apoptosis occurred when the cells were treated with 25 μg/mL and 50μg/mL curcumin for 48 h.When the cells were separately treated with 25μg/mL and 50μg/mL curcumin for 48 h,the relative content of P53 protein was significantly increased compared to control.Conclusion: The data suggested that curcumin might inhibit A549 lung cancer cell proliferation and induce apoptosis through activating P53.