Antitumor Activity of Three Monomer Compounds from Cynanchum Chinense in Vitro

Objective To screen the antitumor activity in vitro of the monomer compounds extracted from the aerial parts of Cynanchum Chinense that is n - octatriacontane(MC -Ⅱ),kaempferol -7 - 0 -α- L-rhamnose glycoside(MC -Ⅴ) and 7-O-α-L- rhamnopyranosyl - kaempferol -3 -O-α-L- rhamnose glycoside(MC -Ⅵ).Methods Three monomer compounds from Cynanchum Chinense Ethyl acetate extraction of purified flavonoid glycosides MC -Ⅴ,alkane MC -Ⅱand n - butanol extraction of purified flavonoid glycosides MC -Ⅵacted respectively on human cervical cancer Hela cells,mouse lymphoma cells YAC -1 and human gastric cancer MGC -803 cell line.After 24h,48h,72h,96h,effects of three monomer compounds on tumor cell proliferation activity were observed by CCK - 8 method.Results Hela cells were treated with MC -Ⅱand the values of OD in different dose groups showed variety from 48h to 72h.The proliferation of YAC -1 cells could be increased by MC -Ⅱat 24h,48h,and 72h while inhibited at 96h. Hela cells and YAC - 1 cells were treated with MC -Ⅴand MC -Ⅵat the concentration of 50,100,and 200μg·mL~(-1) respectively,as a result,the values of OD were lower in the experimental groups than those in control groups(P 0.05) and it showed dose -dependent.Similarly,24h,48h,72h,and 96h after MGC- 803 cells were treated with different dose of MC -Ⅴ,the values of OD were lower in the experimental groups than those in control groups(P 0.05),however,the inhibition effect of MC -Ⅵon MGC -803 cells could be observed only at 24h after treated.Conclusion The growth of tumor cells could be inhibited by the extracts of Cynanchum Chinense flavonoid glycoside MC -Ⅴand MC -Ⅵ.