Expression of Avian Influenza Virus NS1 Protein in Baculovirus Expression Vector System

In order to obtain purified avian influenza virus NS1 protein,NS1gene of A/chicken/Guangxi/10/99(H9N2)(CK/GX/10/99) was cloned into baculovirus transfer vector pFastBacHTA and constructed a recombinant transfer vector pFastBacHTA-NS1.Plasmid pFastBacHTA-NS1 was then transformed into DH10Bac competent cells to obtain recombinant bacmid after the recombinant Bacmid DNA was isolated and transfected into Sf9 insect cells to generate a recombinant NS1 expressing baculovirus which was transfected into Sf9 insect cells to express NS1 protein.The expressed NS1 protein was tested using SDS-PAGE,Western blot and indirect immunofluorescence assay(IFA).The results showed that the recombinant baculovirus containing NS1 gene was constructed successfully and the recombinant protein was successfully expressed with a relative molecular of 30 ku.This work provides the basic material for NS1 related function research.