Apoptosis of human hepatocellular carcinoma cell induced by 12-lipoxygenase inhibitor and effect of 12-lipoxygenase inhibitor on expression of survivin gene

Objective To investigate the effect of 12-lipoxygenase(12-LOX) inhibitor inducing the apoptosis of human hepatocellular carcinoma cell line HepG2 on the expression of survivin gene.Methods HepG2 cells were cultivated in RPMI-1640 medium.12-LOX mRNA was analyzed by reverse transcription polymerase chain reaction(RT-PCR).The effect of baicalein on the proliferation of the cells was detected by thiazolyl blue tetrazoliumbromide(MTT) method.The subcellular structures were observed under electron microscope.DNA ladder pattern on agarose gel electrophores was used to evaluate the apoptotic index of hepG2 cells.The expression of survivin mRNA was analyzed by RT-PCR.Results 12-LOX mRNA was expressed in human HepG2 cells.At concentrations from 20 to 80 μmol·L-1,baicalein inhibited the proliferation of HepG2 cells in a concentration-and time-dependent manner from 24 to 72 h.There were significant differences between any other groups(P0.05).Morphological changes such as chromatin condensatio,apoptotic bodies were observed after treated with baicalein.Significant apoptosis was also induced by baicalein,and the apoptotic index was detected by agarose gel electrophores which showed eveident DNA fragmentation.12-LOX inhibitor depressed the expression of survivin mRNA in a concentration-and time-dependent manner from 24 to 72 h.Conclusion 12-LOX inhibitor can induce the the apoptosis of human hepatocellular carcinoma cell line HepG2,and the probable mechanism is to depress the expression of anti-apoptosis gene survivin.