The Effect of Delisheng in Inducing Apoptosis and Survivin Expression of Human Non-small Cell Lung Cancer A549 Cell in Vitro

Objective To investigate the effect of Delisheng on inducing apoptosis and survivin expression of human non-small cell lung cancer A549 cell.Methods MTT assay was used to determine the proliferation inhibition by Delisheng to A549 cell.Annexin-V kit was used to test the early apoptosis of A549 cells induced by Delisheng.The rate apoptotic cells were assessed by propidium iodide(PI) staining.Semi-quantitative RT-PCR was employed to measure the expression of survivin mRNA.Results(1)4 mL/L～100 mL/L Delisheng significantly inhibited the proliferation of A549 cells in a concentration-independent manner,but in a time-dependent manner(r=0.991,P=0.001).(2)20 mL/L Delisheng induced apoptosis of A549 cells in vitro,and the function was in a time-dependent manner(r=0.997,P=0.049).(3)RT-PCR proved :the related level of survivin mRNA expression was markedly decreased in A549 cells treated with 20 mL/L Delisheng for 24 hours than that in untreated cells(t=4.333,P0.05).Conclusion Delisheng could result in obvious growth inhibitory effects of human non-small cell lung cancer A549 cell and induce apoptosis.the suppressed expression of survivin may be one of the mechanisms of apoptosis induction.