Expression and Identification of Dengue 2 Virus Capsid Protein in Mammalian Cells

The D2C gene fragments were amplified by high fidelity PCR from the constructed plasmid pLEX-C and then cloned into enkaryotic expression vector pcDNA6/V5-His to generate the desired recombinant plasmid pc/D2C.After transfected into the mammalian BHK21 cells by electroporation,the expression of recombinant proteins were identified by RT-PCR,indirect immunofluorescence and Western blot.The results of IFA showed the existence of D2C in the cytoplasm.The results of Western blot indicated the expressed D2C could react with the Mab 8H8 that specifically identified the capsid protein of dengue-2 virus.This work laid the foundation of interests on the biological function of capsid protein during the life cycle of dengue virus.