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2011•Journal of Sun Yat-sen UniversityRequires access

Luteolin Enhances Function of Gap Junction Composed of Cx32/Cx26

LI Li-jun, YU Mei-ling, Suzhi Zhang, Qin Wang, Liang Tao

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Abstract

[Objective]To investigate the effect of luteolin on gap junction intercellular communication and the expression of protein in Cx32/Cx26-transfected Hela cells.[Methods]SRB assay was used to detect the cytotoxicity of luteolin on Hela cells.The function of GJIC was determined by parachute dye-coupling assay.Western blotting was performed to detect the expression level of Cx26.[Results] Luteolin had no cytotoxicity on Hela cells at the concentration of 0～1 μmol/L;Preatreatment of cells with luteolin increased intercellular dye transfer and the Cx26 protein expression.[Conclusion] Luteolin enhanced the function of gap junction in Cx32/Cx26-transfected Hela cells,probably through the improvement of Cx26 expression.

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What this paper is about

[Objective]To investigate the effect of luteolin on gap junction intercellular communication and the expression of protein in Cx32/Cx26-transfected Hela cells.[Methods]SRB assay was used to detect the cytotoxicity of luteolin on Hela cells.The function of GJIC was determined by parachute dye-coupling assay.Western blotting was performed to detect the expression level of Cx26.[Results] Luteolin had no cytotoxicity on Hela cells at the concentration of 0～1 μmol/L;Preatreatment of cells with luteolin increased intercellular dye transfer and the Cx26 protein expression.[Conclusion] Luteolin enhanced the function of gap junction in Cx32/Cx26-transfected Hela cells,probably through the improvement of Cx26 expression.

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Available abstract

[Objective]To investigate the effect of luteolin on gap junction intercellular communication and the expression of protein in Cx32/Cx26-transfected Hela cells.[Methods]SRB assay was used to detect the cytotoxicity of luteolin on Hela cells.The function of GJIC was determined by parachute dye-coupling assay.Western blotting was performed to detect the expression level of Cx26.[Results] Luteolin had no cytotoxicity on Hela cells at the concentration of 0～1 μmol/L;Preatreatment of cells with luteolin increased intercellular dye transfer and the Cx26 protein expression.[Conclusion] Luteolin enhanced the function of gap junction in Cx32/Cx26-transfected Hela cells,probably through the improvement of Cx26 expression.

Key concepts: Luteolin, HeLa, Cytotoxicity, Gap junction, Intracellular, Blot, Transfection, Chemistry

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