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2006•Journal of Dalian UniversityRequires access

Effect of TMP on multidrug resistance reversal and P-gp expression in human breast cancer cell line MCF-7/ADM

Xia Xie, Yang Yi, Gao Qing-bo, Kun Li, Shuang Jin, Na Peng, Hui Xie

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Abstract

[Objective]To investigate the effect of TMP on multidrug resistance reversal and P-glycoprotein(P-gp) expression in human breast cancer MCF-7/ ADM cell line.[Methods] The sensitivity of MCF-7/ADM cells and the reversal of resistance were assessed by MTT assay.P-gp expression was determined by flow cytometry.[Results] The non-cytotoxic dose(320g/ml) and low-cytotoxic dose(320g/ml) of TMP decreased significantly the IC_(50) value of ADM to MCF-7/ADM cells(P0.01),and reversed the resistance by 2.14 folds,2.80 folds respectively compared with the IC_(50) value in MCF-7/ADM and reduced P-gp expression from(90.60±0.40)% to(69.10±1.65)% and(60.30±1.25)% respectively.[Conclusion]: TMP could partially reverse the resistance to ADM in human breast cancer MCF-7/ ADM cell line,which may be related to inhibiting P-gp expression in MCF-7/ADM cells.

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What this paper is about

[Objective]To investigate the effect of TMP on multidrug resistance reversal and P-glycoprotein(P-gp) expression in human breast cancer MCF-7/ ADM cell line.[Methods] The sensitivity of MCF-7/ADM cells and the reversal of resistance were assessed by MTT assay.P-gp expression was determined by flow cytometry.[Results] The non-cytotoxic dose(320g/ml) and low-cytotoxic dose(320g/ml) of TMP decreased significantly the IC_(50) value of ADM to MCF-7/ADM cells(P0.01),and reversed the resistance by 2.14 folds,2.80 folds respectively compared with the IC_(50) value in MCF-7/ADM and reduced P-gp expression from(90.60±0.40)% to(69.10±1.65)% and(60.30±1.25)% respectively.[Conclusion]: TMP could partially reverse the resistance to ADM in human breast cancer MCF-7/ ADM cell line,which may be related to inhibiting P-gp expression in MCF-7/ADM cells.

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Available abstract

[Objective]To investigate the effect of TMP on multidrug resistance reversal and P-glycoprotein(P-gp) expression in human breast cancer MCF-7/ ADM cell line.[Methods] The sensitivity of MCF-7/ADM cells and the reversal of resistance were assessed by MTT assay.P-gp expression was determined by flow cytometry.[Results] The non-cytotoxic dose(320g/ml) and low-cytotoxic dose(320g/ml) of TMP decreased significantly the IC_(50) value of ADM to MCF-7/ADM cells(P0.01),and reversed the resistance by 2.14 folds,2.80 folds respectively compared with the IC_(50) value in MCF-7/ADM and reduced P-gp expression from(90.60±0.40)% to(69.10±1.65)% and(60.30±1.25)% respectively.[Conclusion]: TMP could partially reverse the resistance to ADM in human breast cancer MCF-7/ ADM cell line,which may be related to inhibiting P-gp expression in MCF-7/ADM cells.

Key concepts: MCF-7, Multiple drug resistance, P-glycoprotein, Human breast, Flow cytometry, Cytotoxic T cell, MTT assay, Cell culture

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