Extraction and Determination of the Polysaccharides from Dandelion

The extraction conditions of polysaccharides from dandelion roots were optimized here.The optimal extraction temperature,time,the ratio of solid to liquid and extraction times were 80℃,3 h,1:30 and 2 times,respectively.80% ethanol was used to removal the impurities(monosaccharides and oligosaccharides)and to precipitate polysaccharides,while Sevag method and ligarine were used for deproteinization and degreasing,respectively.The content of polysaccharides was determined by spectrophotometric method with anthrone-sulfuric acid as coloring reagent and shown to be 52.06% with the recovery rate and RSD being of 97% and 2.06%,respectively.The method is suitable for the determination of dandelion polysaccharides due to its simpleness,convenience,accurateness and repeatability.