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2005•Journal of Medical Science Yanbian UniversityRequires access

Construction of fusion expression vector of Ebp 1/EGFP and its expression in HEK293 T cells

Jin Zheng

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Abstract

OBJECTIVE To construct a Ebp 1 and EGFP fusion gene and express the gene in HEK 293 T cells. METHODS The pEGFPC 1-Ebp 1 was constructed by PCR and gene engineering technique,and then the recombinant vector was transfected into HEK 293 T cells by using lipofectamine. RESULTS The HEK 293 T cells were successfully transfected pEGFPC 1-Ebp 1 gene,which confirmed by fluoroscopy.

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What this paper is about

OBJECTIVE To construct a Ebp 1 and EGFP fusion gene and express the gene in HEK 293 T cells. METHODS The pEGFPC 1-Ebp 1 was constructed by PCR and gene engineering technique,and then the recombinant vector was transfected into HEK 293 T cells by using lipofectamine. RESULTS The HEK 293 T cells were successfully transfected pEGFPC 1-Ebp 1 gene,which confirmed by fluoroscopy.

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Available abstract

OBJECTIVE To construct a Ebp 1 and EGFP fusion gene and express the gene in HEK 293 T cells. METHODS The pEGFPC 1-Ebp 1 was constructed by PCR and gene engineering technique,and then the recombinant vector was transfected into HEK 293 T cells by using lipofectamine. RESULTS The HEK 293 T cells were successfully transfected pEGFPC 1-Ebp 1 gene,which confirmed by fluoroscopy.

Key concepts: HEK 293 cells, Lipofectamine, Transfection, Recombinant DNA, Molecular biology, Green fluorescent protein, Fusion gene, Expression vector

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