Relationship Between Serum HBV-DNA Replication and T Lymphocyte Subsets of Peripheral Blood in Patients with Hepatitis B

To observe T lymphocyte subsets in peripheral blood in patients with hepatitis B and the replication of serum hepatitis B virus(HBV-DNA),to explore the relationship between the viral replication degree and cellular immune function.The relative percentage of T lymphocyte subsets in peripheral blood of 142 patients with hepatitis B and 35 healthy controls was analyzed by flow cytometry.The serum load of HBV-DNA was measured by fluorescence quantitative polymerase chain reaction(PCR).Compared with healthy controls,the percentage of CD4+ T cells in patients with chronic hepatitis B were significantly decreased and the percentage of CD8+ T cells were increased(P0.01).Compared with healthy controls,the CD4+/CD8+ratio in patients with cirrhosis were decreased and the percentage of CD4+CD25+ regulatory T cells were increased(P0.05).Chronic hepatitis B infection can lead to dysfunction of cellular immunity;HBV-DNA high replication status can further promote the cellular immune dysfunction.The dynamic detection of CD4+/CD8+ ratio can provide the basis for clinical immunotherapy.