2013•Chinese Journal of Drug Application and MonitoringRequires access
Protective effects of ulinastatin on kidney damage induced by paraquat poisoning in rabbit
Wan Guang-zh
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Abstract
Objective: To study the protective effects of ulinastatin on kidney damage induced by paraquat poisoning in rabbit. Methods: Thirty healthy New Zealand white rabbits were divided into paraquat poisoning group, ulinastatin group and 0.9% sodium chloride solution control group randomly, with 10 rabbits in each group. Paraquat poisoning group and ulinastatin group were given a single dose of paraquat solution 35 mg·kg-1by intraperitoneal injection, control group was given the same volume of 0.9% sodium chloride solution injected in peritoneal cavity. The ulinastatin group received the intravenous injection of ulinastatin100 ku·kg-1after injection of paraquat for 1 hour, then the same dose of ulinastatin was given every day. Dynamic volume CT scan on kidney of each rabbit was given 3 days later for observing the changes of renal blood flow(RBF). The renal tissue pathology was observed, and 2 mL blood was collected from ear marginal vein for detecting serum creatinine(Cr). Results: Compared with the control group and intervention group, RBF decreased obviously and Cr increased significantly in paraquat poisoning group. RBF was higher and Cr was lower in intervention group compared with that of control group. Observed by light microscope, glomerular endothelial cell vacuolar degeneration and necrosis were found in paraquat poisoning group. While in intervention group, the proximal tubular epithelial cells were swelling and vacuolar degeneration, glomerular endothelial cells had no obvious change.Renal organizational structure was clear in control group. Conclusion:Ulinastatin had a protective effect on rabbit kidney damage induced by paraquat poisoning, through improving renal circulation and promoting paraquat elimination.