Influence of the Peiyu particles on invasion and proliferation of human trophoblast cell

Objective To observe the Effect of the Peiyu particles on invasion and proliferation capabilities of human trophoblast cell. Methods Placental villi from normal pregnancy of 5 to 10 weeks were taken to culture trophoblast cells. Twenty percent pharmacological serum obtained from Peiyu particles-administered normal female SD rats( aged23 to 25 days) was added in nutrient solution for culture. After being divided into groups,trophoblast cells were cultured for 48 hours. Then,invasion cell number,absorbance and PCNA expression rate of trophoblast cell in all groups were analyzed by TW invasion assay,MTT assay,and flow cytometry,respectively. Results TW assay results show that invasion cell number of each dose group of Peiyu particles is different from that of the normal group,and the invasion cell number of medium dose group is the highest. MTT assay results show that each dose group of Peiyu particles is different from the normal group in absorbance with absorbance of medium dose group being the highest. Flow cytometry results also show that there are differences between each dose group of Peiyu particles and the normal group in PCNA expression rate,and the PCNA expression rate of medium dose group is the highest. Conclusion Peiyu particles can enhance invasion and proliferation abilities of human trophoblast cell,and its effect may be dose-dependent.