Study on As_2O_3 Regulate to Apoptosis and Fas, FasL Expression of Rat Spermatogenic Cells

Objective To investigate the occurrence of spermatogenic cells apoptosis and the expression of Fas ,FasL after aniso- doses arsenic (As2O3) administration in the testes of the adult male rats. Methods 40 healthy male Sprague-Dawle rats were divided randomly into four groups and they were administered respectively with 0 (control group)、0.375、0.75、1.5 mg/kg of As2O3 by intragastric administration consecutively for 16 weeks. The apoptosis of spermatogenic cells were assessed by in situ terminal deoxynucleotityl transferase mediated dTUP nick end labeling(TUNEL) technique. The expression of Fas and FasL in spermatogenic cells of different grades were located and quantitated by method of immunohistochemistry. Results ①The apoptosis index of spermatogenic cells(AI) significantly increased, compared with that in the control group (P0.01);Fas and FasL expression of middle dose group and high dose group increased significantly,compared with that in the control group (P0.01);②The dependability analysis between DSP and AI showed a negative correlation(r = -0.563,P0.01).③The positive correlation was significant between AI and Fas ,FasL expression of spermatogenic cells(r =0.767, r =0.771,P0.01). Conclusions One mechanisms of male reproduction toxicity of As2O3 might be that Fas and FasL expression is encouraged,which induces decrese of quantity of sperm cells