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Active Efflux Pump MexAB-OprM of Pseudomonas aeruginosa and Its Relationship with Drug-resistance

Congrong Li

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Abstract

OBJECTIVE To study the mechanism of imipenem resistance in clinical isolates of Pseudomonas aeruginosa. METHODS OprM gene is one part of the active efflux pump MexAB-OprM gene, the level of mRNA expressions of OprM in clinical P. aeruginosa isolates was determined by RT-PCR, and rpoD gene as an intrinsic control. RESULTS The levels of mRNA expression of OprM in imipenem-resistant strains were higher than those in susceptible ones(P0.05). CONCLUSIONS The study indicated that the mRNA level of expressions of MexAB-OprM determines the level of imipenem-resistance in P. aeruginosa.

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What this paper is about

OBJECTIVE To study the mechanism of imipenem resistance in clinical isolates of Pseudomonas aeruginosa. METHODS OprM gene is one part of the active efflux pump MexAB-OprM gene, the level of mRNA expressions of OprM in clinical P. aeruginosa isolates was determined by RT-PCR, and rpoD gene as an intrinsic control. RESULTS The levels of mRNA expression of OprM in imipenem-resistant strains were higher than those in susceptible ones(P0.05). CONCLUSIONS The study indicated that the mRNA level of expressions of MexAB-OprM determines the level of imipenem-resistance in P. aeruginosa.

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Available abstract

OBJECTIVE To study the mechanism of imipenem resistance in clinical isolates of Pseudomonas aeruginosa. METHODS OprM gene is one part of the active efflux pump MexAB-OprM gene, the level of mRNA expressions of OprM in clinical P. aeruginosa isolates was determined by RT-PCR, and rpoD gene as an intrinsic control. RESULTS The levels of mRNA expression of OprM in imipenem-resistant strains were higher than those in susceptible ones(P0.05). CONCLUSIONS The study indicated that the mRNA level of expressions of MexAB-OprM determines the level of imipenem-resistance in P. aeruginosa.

Key concepts: Pseudomonas aeruginosa, Efflux, Imipenem, Microbiology, Drug resistance, Pseudomonadaceae, Pseudomonadales, Biology

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