Application of full-automatic hemoglobin analyzer in the screening and diagnosis of thalassemia

Objective To investigate the application of full-automatic hemoglobin analyzer using high performance liquid chromatography(HPLC) in the screening and diagnosis of thalassemia.Methods The hemoglobin of 5738 testing samples were analyzed by using full-automatic hemoglobin analyzer and its supplementary reagents.830 samples screened out with abnormal results were conducted gene detection of thalassemia by using multiplex polymerase chain reaction(PCR) and reverse dot blot(RDB),and its coincidence rate was compared.Results In the 5738 subjects,830 cases were abnormal with a detectable rate of 14.46%,and 480 cases of them were suspected as α thalassemia(57.83%),316 cases were suspected as β thalassemia(38.07%),11 cases were suspected as β compound α thalassemia(1.33%).13 cases detected abnormal zone with HbH,HbE in 8 cases,HbQ in 2 cases,respectively accounting for 1.57%,0.96% and 0.24%.After thalassemia gene analysis,743 patients were diagnosed with diagnosis rate of 89.52%.413 cases were α thalassemia(55.59%),the coincidence rate was 86.04%.313 cases were β thalassemia(42.13%),the coincidence rate was 99.05%.17 cases were β compound α thalassemia(2.29%) with the coincidence rate of 90.91%.Conclusion The full-automatic hemoglobin analyzer using HPLC technology has a good compliance in the diagnosis of β thalassemia,and it has certain assistance in the diagnosis of α thalassemia.It can be used for the screening and diagnosis of thalassemia.