2012•Acta Agrestia SinicaRequires access

Establishment and Optimization of ISSR Reaction System for Pea

Farong Yang

Abstract

The optimum ISSR-PCR system for peas is established using ISSR-PCR amplification with five factors(DNA template,primer,dNTPs,Taq DNA polymerase and annealing temperature).The optimum reaction system(20 μL) contains 15 ng DNA template,0.15 mmol·L-1 dNTP,0.5 μmol·L-1 ISSR primer,1U Taq DNA polymerase.The suitable annealing temperature of primer 842 is 50℃.This reaction system will provide a technological base for genetic diversity analysis of pea germplasm,genetic map construction and gene location.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Germplasm, Primer (cosmetics), Polymerase chain reaction, DNA, Genetic diversity, Taq polymerase, Biology, DNA profiling