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Study on the genotyping of human platelet antigen-2,-4,-5 system by multiplex polymerase chain reaction

Shen Jiji

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Abstract

Objective To develop a method for genotyping of the human platelet antigen(HPA)-2,-4,-5 system by means of multiplex polymerase chain reaction(PCR) technique. Methods HPA-2,-4,-5 antigen system genes were simultaneously amplified in one amplification system.The results of genotyping HPA-2,-4,-5 antigen system by multiplex PCR were compared with those obtained by PCR-sequence specific primer(SSP). Results The genotype of HPA-2,-4,-5 antigen system obtained by multiplex PCR in 75 healthy platelet donors were 70 of 2a/2a,5 of 2a/2b,0 of 2b/2b;73 of 4a/4a,2 of (4a/4b,) 0 of 4b/4b;66 of 5a/5a,9 of 5a/5b and 0 of 5b/5b.All were in agreement with those determined by PCR-SSP. Conclusions The method of multiplex PCR is convenient,economical,rapid,and accurate.It may be appropriate for genotyping of HPA.

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Objective To develop a method for genotyping of the human platelet antigen(HPA)-2,-4,-5 system by means of multiplex polymerase chain reaction(PCR) technique. Methods HPA-2,-4,-5 antigen system genes were simultaneously amplified in one amplification system.The results of genotyping HPA-2,-4,-5 antigen system by multiplex PCR were compared with those obtained by PCR-sequence specific primer(SSP). Results The genotype of HPA-2,-4,-5 antigen system obtained by multiplex PCR in 75 healthy platelet donors were 70 of 2a/2a,5 of 2a/2b,0 of 2b/2b;73 of 4a/4a,2 of (4a/4b,) 0 of 4b/4b;66 of 5a/5a,9 of 5a/5b and 0 of 5b/5b.All were in agreement with those determined by PCR-SSP. Conclusions The method of multiplex PCR is convenient,economical,rapid,and accurate.It may be appropriate for genotyping of HPA.

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Available abstract

Objective To develop a method for genotyping of the human platelet antigen(HPA)-2,-4,-5 system by means of multiplex polymerase chain reaction(PCR) technique. Methods HPA-2,-4,-5 antigen system genes were simultaneously amplified in one amplification system.The results of genotyping HPA-2,-4,-5 antigen system by multiplex PCR were compared with those obtained by PCR-sequence specific primer(SSP). Results The genotype of HPA-2,-4,-5 antigen system obtained by multiplex PCR in 75 healthy platelet donors were 70 of 2a/2a,5 of 2a/2b,0 of 2b/2b;73 of 4a/4a,2 of (4a/4b,) 0 of 4b/4b;66 of 5a/5a,9 of 5a/5b and 0 of 5b/5b.All were in agreement with those determined by PCR-SSP. Conclusions The method of multiplex PCR is convenient,economical,rapid,and accurate.It may be appropriate for genotyping of HPA.

Key concepts: Genotyping, Multiplex polymerase chain reaction, Multiplex, Polymerase chain reaction, Primer (cosmetics), Molecular biology, Antigen, Genotype

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