Determination of Diallyl Disulfide and Diallyl Trisulfide in Allium Sativum L. from Difference Places

Objective To study the diallyl disulfide and diallyl trisulfide content in Allium sativum L. from different places. Method C18 column, mobile phase was methanol-0.17% Formic acid (80∶20). The detection wavelength was at 240 nm and the flow rate was 1.0 mL/min. Result The linearity was obtained at the range of 0.368~7.36 μg (r=0.9999) for diallyl disulfide, 0.2288~4.576 μg (r=0.9999) for diallyl trisulfide. Conclusion The contents of diallyl disulfide and diallyl trisulfide in Allium sativum L. from different places are very different. We should have a choice in use.