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IN VITRO INDUCTION OF POLYPLOIDY FROM COLCHICINE-TREATED ZINGIBER OFFICINALE ROSCSE

Guo Qigao

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Abstract

An experiment was made to induce polyploidy in ginger ( Zingiber officinale Roscse) with 5 methods of colchicine treatment.Best results for inducing tetraploid plantets were obtained when the explants were cultured for 5 days in a medium supplemented with colchicine at 30 mg/L,the induction frequency being 65.0%. Induction frequency was 27.5%,22.5% and 47.5%,respectively, when the leaves were stripped and the bare growth tips were treated with 0.1% colchicine by injection, soaking and embedding with adsorbent cotton.No tetraploid plantets were differentiated in the treatment of colchicine dropping.

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What this paper is about

An experiment was made to induce polyploidy in ginger ( Zingiber officinale Roscse) with 5 methods of colchicine treatment.Best results for inducing tetraploid plantets were obtained when the explants were cultured for 5 days in a medium supplemented with colchicine at 30 mg/L,the induction frequency being 65.0%. Induction frequency was 27.5%,22.5% and 47.5%,respectively, when the leaves were stripped and the bare growth tips were treated with 0.1% colchicine by injection, soaking and embedding with adsorbent cotton.No tetraploid plantets were differentiated in the treatment of colchicine dropping.

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Available abstract

An experiment was made to induce polyploidy in ginger ( Zingiber officinale Roscse) with 5 methods of colchicine treatment.Best results for inducing tetraploid plantets were obtained when the explants were cultured for 5 days in a medium supplemented with colchicine at 30 mg/L,the induction frequency being 65.0%. Induction frequency was 27.5%,22.5% and 47.5%,respectively, when the leaves were stripped and the bare growth tips were treated with 0.1% colchicine by injection, soaking and embedding with adsorbent cotton.No tetraploid plantets were differentiated in the treatment of colchicine dropping.

Key concepts: Colchicine, Zingiber officinale, In vitro, Explant culture, Botany, Biology, Traditional medicine, Horticulture

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