ScholarLens Back to search
2007•Food ScienceRequires access

Determination of Chlorogenic Acid in Eucommia Ulmoides Olive Leaves by Reversed-Phase High Performance Liquid Chromatography

Bing Yan

Open publisher page 0 citations

Abstract

The conditions for the extracting chlorogenic acid in the Eucommia Ulmoides Olive leaves were optimized by the orthogonal test as follows: continuous extraction by 30% methanol for 20 minutes; determined by RP-HPLC. The chlorogenic acid was seperated on kromasil C18 column, with CH3OH～0.02%H3PO4 (22:78) at a flow-rate of 1.0ml/min and detected at UV 327nm.The calibration curve was linear in the range of 9.68～242μg/ml (r=0.9999). This method is simple, rapid and accurate with good separation, and the recoveries are above 95.9%.

About this research paper

What this paper is about

The conditions for the extracting chlorogenic acid in the Eucommia Ulmoides Olive leaves were optimized by the orthogonal test as follows: continuous extraction by 30% methanol for 20 minutes; determined by RP-HPLC. The chlorogenic acid was seperated on kromasil C18 column, with CH3OH～0.02%H3PO4 (22:78) at a flow-rate of 1.0ml/min and detected at UV 327nm.The calibration curve was linear in the range of 9.68～242μg/ml (r=0.9999). This method is simple, rapid and accurate with good separation, and the recoveries are above 95.9%.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The conditions for the extracting chlorogenic acid in the Eucommia Ulmoides Olive leaves were optimized by the orthogonal test as follows: continuous extraction by 30% methanol for 20 minutes; determined by RP-HPLC. The chlorogenic acid was seperated on kromasil C18 column, with CH3OH～0.02%H3PO4 (22:78) at a flow-rate of 1.0ml/min and detected at UV 327nm.The calibration curve was linear in the range of 9.68～242μg/ml (r=0.9999). This method is simple, rapid and accurate with good separation, and the recoveries are above 95.9%.

Key concepts: Eucommia ulmoides, Chlorogenic acid, Chromatography, High-performance liquid chromatography, Phosphoric acid, Calibration curve, Chemistry, Extraction (chemistry)

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Chlorogenic Acid in Eucommia Ulmoides Olive Leaves by Reversed-Phase High Performance Liquid Chromatography — Research Paper | ScholarLens