Construction and identification of recombinant vector pLXSN-EGFP-IRES2-GDNF

【Objective】 To construct and confirm a recombinant retroviral vector carrying human GDNF gene.【Methods】 GDNF gene was amplified by PCR from glioma of human brain tissues.The gene was cloned into a retroviral vector pLXSN and recombinant vector plasmid DNA was analyzed after digested by appropriate restriction enzymes.【Results】 The correct sequence of human GDNF was obtained after compared with the sequence in GenBank.The recombinant vector pLXSN-EGFP-IRES2-GDNF was successfully constructed after confirmed by enzyme digestion analysis.【Conclusions】 Construction of eukaryotic cell expression retroviral vectors carrying human GDNF gene is feasible.It lays a foundation for further research in gene therapy of nervous system disease.