Analysis of multi-genotypes of ESBLs coexistent in clinical isolates of Klebsiella pneumoniae

Objective To find the multi-genotypes of extended-spectrum β-lactamase(ESBLs) in clinical isolates of Klebsiella pneumoniae in the First Affilated Hospital,Shantou University Medical Colloege.Methods ESBLs genotypes were performed by PCR,and the PCR products were cloned and sequenced.DNA analysis was by pulsed-field gel electrophresis(PFGE).The MICs of 10 antibiotics were detected by broth-dilution against the multi-genotypes of ESBLs coexistent in clinical isolates of Klebsiella pneumoniae.Results The results of PCR showed that among the 83 strains clinical isolates of Klebsiella pneumoniae producing ESBLs,there were 3-geneotypical ESBLs coexistent in 9 strains.They were confirmed to CTX-M-3.TEM-1 as well as SHV genotypes.The DNAs analysis by PFGE in these 9 strains showed that there were 7 different types.These 9 strains were high resistance to ATM,CRO,CTX,CAZ,AKN and TET except IMP,but a few susceptive to TZP,FEP and CIP.Conclusion There are CTX-M-3 and SHV-extended-spectrum β-lactamase coexistent with TEM-1 broad-spectrum β-lactamase in 9 strains clinical isolates of Klebsiella pneumoniae in the hospital.They were seriously resistance to drugs.