2012•Journal of environmental healthRequires access
Application of Conventional Culture and Real Time PCR Method in Detection of Legionella in Shower Warm Water
Ran Zhang, Chen GuiBing, Lin AiHong
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Abstract
Objective To detect Legionella in shower warm water by conventional culture method and real-time fluorescent PCR.Methods According to the Sanitation Criteria for Central Conditioners in Public Buildings,a total of 86 samples of shower warm water were detected,isolated and cultured with conventional method in October 2010-December 2011.Real time fluorescent PCR method was used to confirm the results.Results A total of 20 strains of Legionella pneumophila and a strain of non-Legionella pneumophila were found in the 86 samples.The positive rate of Legionella pneumophila was 23.3%,Lp1,Lp3,Lp6 were 65%,10%,25%.Conclusion Legionella exists in shower warm water at hotels in Shenzhen.The conventional culture and real-time fluorescent PCR has high consistency.