Isolation and characterization of microsatellite loci of Siniperca chuatsi from GenBank database

This project made use of bioinformatic mining of microsatellites from genomic resources to identify simple sequence repeats(SSRs),or microsatellites.A bioinformatic analysis of 304 nucleotide sequences in Siniperca chuatsi GenBank identified 22 sequences containing 30 microsatellites,account for 9.87% of wholly GenBank database.Cluster analysis indicated that 16 dinucleotide pairs were the most abundant microsatellites,accounting for 53.33% of the total microsatellite-containing sequences;11 trinucleotide repeats and 3 tetranucleotide repeats were found in these microsatellite sequences,accounting for 36.66% and 10.01% respective.17 primer pairs were designed from these microsatellite sequences.Among the 17 primer pairs,13 pairs have amplified products,6 pairs can achieve clear PCR products by Electrophoresis.Genotype analysis results indicated that:the average polymorphism information contents(PIC)were 0.692(ranged:0.567-0.806),were all polymorphic loci.