Study on Chemical Constituents of Root of Smilax scobinicaulis

OBJECTIVE To study the fat-soluble constituents in the root of Smilax scobinicaulis.METHODS Various isolation and identification techniques such as silica gel,RP-18,polydextran gel(Sephadex LH-20),semi-preparation HPLC were used.The structures were elucidated on the basis of spectral data and chemical properties.RESULTS Thirteen compounds were isolated from the ethyl acetate extract of the alcohol extract of Smilax scobinicaulis C.H.Wright root,and identified as: β-sitosterol(1),25R-3β-hydroxy-5α-spirostan-6-one(2),clemaphenolA(3),2,4-dihydroxy-3,6-dimethylbenzoate(4),ursolic acid(5),blumenol A(6),1-O-p-coumaroylglycerol(7),1-O-feruloylglycerol(8),quercetin(9),luteolin-7-O-β-D-glucoside(10),trans-resveratrol(11),quercetin-3-O-β-D-glucpside(12),daucosterolon(13)the basis of NMR data and comparison with literatures.CONCLUSION Compounds 3-8 and 10-12 are for the first time isolated from Smilax scobinicaulis C.H.Wright.