The clinical application of detection drug-resistant mutational site and HBV genotype

Objective To establish a direct gene sequencing method of HBV DNA P gene and the genotype,explore the relationship between the HBV genotype and pathogetic condition.Methods46 HBV infected serum samples were detected.HBV DNA P genes were amplified by PCR and sequenced.HBV mutation were identified by Chromas 2.23 compared with standard sequence,genetype were analysed by clustalx 1.81 software.ResultsThe genotypic and mutational HBV could be detected successfully.Of 46 serum speciments,there were 23 samples belong to genotype B(50%),21 genotype C(45.6%) and 2 genotype D(4.4%).And there were 2 hepatocellular carcinoma(8.7%) in genotype B patiens,8 hepatocellular carcinoma(38.1%) in genotype C patients.ConclusionDirect gene sequencing method is a feasible,accurate method for clinical detection of HBV drug-resistant mutational site and genotype.The prognosis of genotype B patients is better than genotype C patients.