The Property of Egr-1 Promoter in Irradiation-Induced Gene Expression

Objective: To study the effect of ionizing radiation on the expression of EGFP reporter gene induced by Egr-1 promoter in human colorectal adenocarcinoma cell line SW480.Methods: The plasmid Egr-1-EGFP was constructed by gene recombination,and was transfected into the SW480 cells with cationic liposome,then the cells were treated with various doses of γ-ray irradiation.FACS was used to detect the EGFP expression at different times after exposure.Results: FACS analysis showed that ionizing radiation could induce the expression of EGFP report gene,and this expression reached maximum at 24 h post 6 Gy γ-ray exposure.Conclusion: Egr-1 gene promoter has ionizing radiation-inducible characteristics.