Taq ManDuplex Real-time Fluorescence PCR Method in Detecting Enterohemorrhagic Escherichia Coli

Objective To evaluate the feasibility of the TaqMan duplex real-time fluorescence PCR assay method for detection of Enterothemorrhagic Escherichia coli.Methods Simulated samples and 295 stools collected from patients with diarrhea were respectively detected by traditional culture method,GB PCR method and the TaqMan duplex real-time fluorescence PCR method,and the three methods were compared in aspects of the detection limit and detection rate.Results The detection limit of the TaqMan duplex real-time fluorescence PCR method was 102-108cfu / ml,which was much better than the traditional method(103-108cfu / ml) and GB PCR method(103-107cfu / ml).The detection rate was 0.34%.Conclusion The TaqMan duplex real-time PCR assay,with characterized by simple,rapid,sensitive,and effective,is suitable for EHEC strain detection.