ScholarLens Back to search
2003•MycosystemaRequires access

PROTOPLAST ISOLATION AND REGENERATION OF MORCHELLA

Cui Zong

Open publisher page 1 citations

Abstract

For the protoplast isolation of Morchella, the optimal age of mycelia was 3d and 0.6ml/L sucrose was the best osmotic stabilizer. The optimal enzymolysis temperature was 30℃and optimal enzymolysis time was 3h. The highest protoplast yield was 3.35×106/100mg. When 0.6mol/L sucrose was used for stabilizer of the regeneration medium of CYM, the regeneration frequency of protoplast was 0.171%. These results and data would provide important parameters for the strain improvement of Morchella through the protoplast technique.

About this research paper

What this paper is about

For the protoplast isolation of Morchella, the optimal age of mycelia was 3d and 0.6ml/L sucrose was the best osmotic stabilizer. The optimal enzymolysis temperature was 30℃and optimal enzymolysis time was 3h. The highest protoplast yield was 3.35×106/100mg. When 0.6mol/L sucrose was used for stabilizer of the regeneration medium of CYM, the regeneration frequency of protoplast was 0.171%. These results and data would provide important parameters for the strain improvement of Morchella through the protoplast technique.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

For the protoplast isolation of Morchella, the optimal age of mycelia was 3d and 0.6ml/L sucrose was the best osmotic stabilizer. The optimal enzymolysis temperature was 30℃and optimal enzymolysis time was 3h. The highest protoplast yield was 3.35×106/100mg. When 0.6mol/L sucrose was used for stabilizer of the regeneration medium of CYM, the regeneration frequency of protoplast was 0.171%. These results and data would provide important parameters for the strain improvement of Morchella through the protoplast technique.

Key concepts: Protoplast, Sucrose, Mycelium, Regeneration (biology), Biology, Yield (engineering), Isolation (microbiology), Osmotic pressure

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
PROTOPLAST ISOLATION AND REGENERATION OF MORCHELLA — Research Paper | ScholarLens