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2009•Journal of Jiangxi University of Traditional Chinese MedicineRequires access

Determination of the Alkaloids in Diferently Processed Coptis Chinensis Franch by RP-HPLC

Yang Jin

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Abstract

Objective:To establish a HPLC method for separation and determination of the alkaloids in diferently processed Coptis chinensis Franch.Methods: HPLC system consisted of C18 column(4.6 mm x 200 mm,5 μm),mobile phase was composed of acetonitrile solution of potassium phosphate(0.05 mol/L,28∶72,adjusting pH value with phosphoric acid to 3.0),and the detection wavelength was 345 nm.Results:Three kinds of berberine—type alkaloids which arejatrorrhizine,epiberberine and berberine could be separated completely.Conclusion:HPLC is of high sensitivity and good reproducibility.It can be used for quality control of drug and Chinese crude drug containing alkaloids.

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Objective:To establish a HPLC method for separation and determination of the alkaloids in diferently processed Coptis chinensis Franch.Methods: HPLC system consisted of C18 column(4.6 mm x 200 mm,5 μm),mobile phase was composed of acetonitrile solution of potassium phosphate(0.05 mol/L,28∶72,adjusting pH value with phosphoric acid to 3.0),and the detection wavelength was 345 nm.Results:Three kinds of berberine—type alkaloids which arejatrorrhizine,epiberberine and berberine could be separated completely.Conclusion:HPLC is of high sensitivity and good reproducibility.It can be used for quality control of drug and Chinese crude drug containing alkaloids.

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Available abstract

Objective:To establish a HPLC method for separation and determination of the alkaloids in diferently processed Coptis chinensis Franch.Methods: HPLC system consisted of C18 column(4.6 mm x 200 mm,5 μm),mobile phase was composed of acetonitrile solution of potassium phosphate(0.05 mol/L,28∶72,adjusting pH value with phosphoric acid to 3.0),and the detection wavelength was 345 nm.Results:Three kinds of berberine—type alkaloids which arejatrorrhizine,epiberberine and berberine could be separated completely.Conclusion:HPLC is of high sensitivity and good reproducibility.It can be used for quality control of drug and Chinese crude drug containing alkaloids.

Key concepts: Coptis chinensis, Chromatography, Berberine, Coptis, Phosphoric acid, High-performance liquid chromatography, Chemistry, Coptisine

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