Influence of miR-92b on cell cycle and apoptosis of gastric cancer cell

Objective To investigate the influence of miR-92b on cell cycle and apoptosis of gastric cancer cell.Methods MiR-92b was inhibited in gastric cancer cell line SGC-7901 through the transient transfection of chemically modified microRNA inhibitors.The changes in cell cycle and apoptosis were detected by flow cytometry and the cell morphology observed under the fluorescence microscope,the changes in Bcl-2 and p53 were identified by western blot.Results After the inhibition of miR-92b,the cell cycle was delayed,the apoptosis rate of gastric cancer cell line increased,the cell nucleus shrinked,and the expression of Bcl-2 and p53 was down-regulated.Conclusion MiR-92b may serve an oncogene function by pushing gastric cancer cells through restriction points,accelerating their growth and promoting their proliferation through inhibited mitochondrial apoptosis pathway.