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2008•Journal of Anhui Science and Technology UniversityRequires access

Optimization of Submerged Fermentation Conditions for Intracellular Polysaccharide of Pleurotus nebrodensis

Ping Wu

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Abstract

Optimization of liquid culture conditions to intracellular polysaccharide was carried out with intracellular polysaccharide as target.According to single test and the orthogonal design,the results showed that the fermentation condition was glucose 3.0%,peptone 0.05%,wheat bran 4.0%,KH2PO4 0.1%,MgSO4 0.1%,shaking rate 160r/min,initial pH 6.5,inoculation rate 10%,volume 80mL/250mL.The intracellular polysaccharide production reached the highest 95.56mg/100mL after cultured 6d,at the temperature of 25℃.

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What this paper is about

Optimization of liquid culture conditions to intracellular polysaccharide was carried out with intracellular polysaccharide as target.According to single test and the orthogonal design,the results showed that the fermentation condition was glucose 3.0%,peptone 0.05%,wheat bran 4.0%,KH2PO4 0.1%,MgSO4 0.1%,shaking rate 160r/min,initial pH 6.5,inoculation rate 10%,volume 80mL/250mL.The intracellular polysaccharide production reached the highest 95.56mg/100mL after cultured 6d,at the temperature of 25℃.

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Available abstract

Optimization of liquid culture conditions to intracellular polysaccharide was carried out with intracellular polysaccharide as target.According to single test and the orthogonal design,the results showed that the fermentation condition was glucose 3.0%,peptone 0.05%,wheat bran 4.0%,KH2PO4 0.1%,MgSO4 0.1%,shaking rate 160r/min,initial pH 6.5,inoculation rate 10%,volume 80mL/250mL.The intracellular polysaccharide production reached the highest 95.56mg/100mL after cultured 6d,at the temperature of 25℃.

Key concepts: Polysaccharide, Bran, Intracellular, Fermentation, Food science, Volume (thermodynamics), Inoculation, Extracellular polysaccharide

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