Establishment of multiplex PCR for rapid detection of porcine pseudorabies virus,porcine circovirus type II and porcine parvovirus

According to the gene sequences in GenBank of PRV gB,PCV2 ORF2,PPV VP2,specific primers for each of three common DNA viruses were designed by Primer Premier 5.0 and DNAstar software,and three specific bands,respectively,PRV( 373 bp),PCV2( 430 bp),PPV( 495 bp) were amplified. It was shown that mPCR was able to detect as little as 1.62 × 10-6,1.47 × 10-6and 1.28 × 10-4ng of each viral target through the highly sensitive and specificity assay. And detections of ddH 2 O,PRRSV, SV were negative at the same time. In conclusion,mPCR would be useful in routine molecular diagnosis and epidemiology of PRV, PCV2 and PPV.