Regulation effect of nerve growth factor on the airway inflammation in TNF-α expression in the lung of the asthmatic rats

Objective To explore the role of nerve growth factor(NGF) in the pathogenesis of asthma and the regulation of nerve growth factor on airway inflammation TNF-α mRNA expression of the possible mechanism.Methods The experiment was divided into control,asthma,NGF blocking groups.Immunohistochemical method combined with microscopic image analysis were used to detect the expression of NGF,and TNF-α mRNA protein expression.Results Compared with the NGF blocking group and the asthma group,the number of asthma attacks and eosinophil count were decreased significantly(all P0.01),bronchial inflammation in lung tissue was significantly reduced.The NGF mRNA expression in the asthmatic lung tissue was significantly higher than that of control group(P0.05),the NGF blocking group which TNF protein in lung tissue and the expression of TNF-α mRNA were significantly were reduced compared with asthma group(P0.05).Conclusions NGF can promote airway inflammation.Blocking NGF can reduce lung eosinophil infiltration,the number of asthma attacks and TNF-α mRNA expression in the asthma rats,and the airway inflammation can be depressed by the intervention of anti-NGF.