Role of RhoA Protein on Regulating the Migration of Vascular Endothelial Cells Induced by IL-8

Objective To investigate the role of RhoA Protein in the migration of endothelial cell. Methods Firstly, endothelial cells were transfected to obtain the wild type RhoA(RhoAwt), the constitutively active forms of RhoA(RhoA63L), and the dominant negative forms of RhoA (RhoA188A), respectively, with lipofectamine 2000 reagent. Secondly, with Western blot technology, the content of activated RhoA in each type of these cells and the control group cells was measured respectively. Finally, with stimulation of IL-8 on these cells, the ability of cell migration was detected, and the interaction between cell migration and activated RhoA was analyzed. Results 1) The content of activated RhoA in each infected group was different with that in the control group. Content of EA.hy926-RhoAwt group was little higher, while EA.hy926-RhoA63L group was great higher, however, EA.hy926-RhoA188A group was lower.2) The ability of cell migration of EA.hy926-RhoAwt group was little higher, EA.hy926-RhoA63L was great higher, and EA.hy926-RhoA188A was inhibited obviously. The relation between ability of cell migration and content of activated protein RhoA was positive. Conclusion It is suggested that RhoA is a key factor in cellular signal transduction process of IL-8 inducing endothelial cell migration. These results will be helpful for investigating the mechanism of IL-8 inducing endothelial cell migration,and also for clinical application.