Effects of Angelica dahurica active extracts on biological behavior of human umbilical vein endothelial cells

Objective To observe the effects of Angelica dahurica extract on the biological characteristics of human umbilical vein endothelial cells(HUVECs) in vitro and to preliminarily explore the possible therapeutic mechanism on inducing wound healing.Methods Cultured human umbilical vein endothelial cells(HUVECs),different concentrations of the Angelica dahurica extracts ware added to the medium.HUVECs were treated with different concentration of Angelica dahurica extracts.The proliferation activity of HUVECs was measured by using thiazole blue(MTT) colorimetric assay.The cyclin D1,Caspase 3 and VEGF mRNA levels of treated HUVECs were detected by real-time polymerase chain reaction(PCR) techniques.Results The Angelica dahurica extracts(at the concentration from 5×10-3g/L~5×10-2g/L) can significantly enhance HUVECs proliferation.The effect of enhancing proliferation was the most significantly at the concentration of 5×10-2g/L(t=3.03,P 0.01).Real-Time PCR revealed that compared with control group,the cyclin D1 and Caspase3 mRNA level in the concentration of 5×10-3g/L Angelica dahurica extract group was significantly down-regulated,VEGF mRNA level no significantly different.Conclusions Angelica dahurica extract can notably promote the proliferation of HUVECs,accelerate the cell cycle of HUVECs and down-regulate the mRNA expression of Caspase 3.These effects may related with the mechanism of Angelica dahurica extract on enhancing the process of wound healing.