ScholarLens Back to search
2004•ACTA MICROBIOLOGICA SINICARequires access

Secreted Overexpression of Alkali-tolerant Xylanase Gene in Bacillus pumilus

Wei Liu

Open publisher page 0 citations

Abstract

The xylanase encoding gene xynA was cloned from Bacillus pumilus BP51, a xylanase over producer. The xynA gene was inserted in Bacillus expression vector pWH1520, downstream of xylA promoter.The recombinant plasmid pWSX11 was transformed back into the original strain B.pumilus BP51 and the recombinant B.pumilus BPx11 was obtained.The xynA was over expressed and the xylanase was secreted into medium. The xylanase activity produced by recombinant BPX11 had increased about 87% as compared with the original strain BP51 The properties of the recombinant xylanase were characterized.

About this research paper

What this paper is about

The xylanase encoding gene xynA was cloned from Bacillus pumilus BP51, a xylanase over producer. The xynA gene was inserted in Bacillus expression vector pWH1520, downstream of xylA promoter.The recombinant plasmid pWSX11 was transformed back into the original strain B.pumilus BP51 and the recombinant B.pumilus BPx11 was obtained.The xynA was over expressed and the xylanase was secreted into medium. The xylanase activity produced by recombinant BPX11 had increased about 87% as compared with the original strain BP51 The properties of the recombinant xylanase were characterized.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The xylanase encoding gene xynA was cloned from Bacillus pumilus BP51, a xylanase over producer. The xynA gene was inserted in Bacillus expression vector pWH1520, downstream of xylA promoter.The recombinant plasmid pWSX11 was transformed back into the original strain B.pumilus BP51 and the recombinant B.pumilus BPx11 was obtained.The xynA was over expressed and the xylanase was secreted into medium. The xylanase activity produced by recombinant BPX11 had increased about 87% as compared with the original strain BP51 The properties of the recombinant xylanase were characterized.

Key concepts: Bacillus pumilus, Xylanase, Recombinant DNA, Gene, Strain (injury), Plasmid, Chemistry, Microbiology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Secreted Overexpression of Alkali-tolerant Xylanase Gene in Bacillus pumilus — Research Paper | ScholarLens