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2011•Journal of Zhejiang Sci-Tech UniversityRequires access

Regulating the Expression of DNA Methyltransferase in Liver Cancer Cells by miR-29b

Qijun Qian

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Abstract

Abnormal DNA methylation plays an extremely important role in tumors.DNA methylation is completed through DNA methyltransferases(DNA methyhransferases,DMNTs).The miR-29 had a certain relationship with the expression of methylation transferase.In this study,an adenovirus vector carrying miR-29b is constructed and the virus is used to infect the liver cancer cell PLC.Then qRT-PCR is exploited to detect the relative quantity of miR-29b and methyltransferase in the infected PLC cells.The results show that the adenovirus carrying miR-29b can effectively elevate the cellular miR-29b and inhibit the expression of methyltransferases.

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What this paper is about

Abnormal DNA methylation plays an extremely important role in tumors.DNA methylation is completed through DNA methyltransferases(DNA methyhransferases,DMNTs).The miR-29 had a certain relationship with the expression of methylation transferase.In this study,an adenovirus vector carrying miR-29b is constructed and the virus is used to infect the liver cancer cell PLC.Then qRT-PCR is exploited to detect the relative quantity of miR-29b and methyltransferase in the infected PLC cells.The results show that the adenovirus carrying miR-29b can effectively elevate the cellular miR-29b and inhibit the expression of methyltransferases.

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Available abstract

Abnormal DNA methylation plays an extremely important role in tumors.DNA methylation is completed through DNA methyltransferases(DNA methyhransferases,DMNTs).The miR-29 had a certain relationship with the expression of methylation transferase.In this study,an adenovirus vector carrying miR-29b is constructed and the virus is used to infect the liver cancer cell PLC.Then qRT-PCR is exploited to detect the relative quantity of miR-29b and methyltransferase in the infected PLC cells.The results show that the adenovirus carrying miR-29b can effectively elevate the cellular miR-29b and inhibit the expression of methyltransferases.

Key concepts: Methyltransferase, DNA methyltransferase, DNA methylation, Methylation, DNA, Biology, Cancer research, Molecular biology

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